GABAergic Neurons
GABA作動性神経細胞
ヒトiPS細胞由来ドーパミン作動性神経細胞
GABA作動性神経細胞分化誘導キット
特長
- 約1週間で分化が完了
- 高純度な細胞集団
- ゲノムに影響なし（フットプリントフリー）
キャラクタライゼーション
分化誘導キット
ワークフロー
Quick-Neuron™ GABAergic - mRNA Kitで誘導開始後1～11日目の代表的な位相差画像（スケールバー＝100μm）。
Quick-Neuron™ GABAergic Neuronの免疫染色
Quick-Neuron™ GABAergic -mRNAキットで誘導開始後11日目の細胞は、神経細胞マーカーTUBB3およびGABA作動性神経細胞特異的マーカーGAD65/67を発現し、典型的な神経突起の伸長を示します（スケールバー=100μm）。
染色条件：Anti-β-III tubulin monoclonal antibody (R&D Sys- tems, Catalog Number: MAB1195, 1:250 希釈)、二次抗体(Invitrogen,Catalog Number: A32723 Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, AlexaFluor Plus 488, 1:500 希釈)
Anti-GAD65/GAD67 primary antibody (Fisher Scientific, Catalog Number: PA5-36080, 1:400 希釈)、二次抗体(Invitrogen, Catalog Number: A11037 Goat anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Sec- ondary Antibody, Alexa Fluor 594, 1:500 希釈)
核はHoechst33342で対比染色を行いました。
GABA作動性神経細胞分化誘導キット
Quick-Neuron™ GABAergic - mRNA Kitsでは、お手持ちのiPS/ES細胞株を高速、簡単、かつ効率的にGABA作動性神経細胞に分化させることができます。 このキットでは、分化を誘導するための弊社独自の転写因子カクテルを合成mRNA(syn-mRNA)で導入するため、フットプリントフリーで、つまりゲノムに影響を与えずに目的の細胞へと分化させることが可能です。
| Cat No. | Product Name | Disease StatusDescription |
|---|---|---|
| GA-mRNA | Quick-Neuron™ GABAergic - mRNA Kit | This kit differentiates human pluripotent stem cells into GABAergic neurons in 10 days using synthetic mRNA. |
| GA-MM | Quick-Neuron™ GABAergic - Maintenance Medium | Medium for long-term maintenance of human iPS/ESC-derived GABAergic neurons. |
iPSC-derived GABAergic Neurons
当社のCholinergic - Human iPSC-derived Neuronsは、カリフォルニア再生医療機構(CIRM, California Institute for Regenerative Medicine )とのライセンス契約に基づき、CIRM保有のヒトiPS細胞株を使用して生産および販売しております。
CIRMのiPS細胞バンクには1,500以上のiPS細胞株が登録されていますので、特定のドナー細胞株由来のコリン作動性神経細胞をご希望の場合はお問い合わせください。当社が提供するコリン作動性神経細胞はすべて当社独自の転写因子ベースの技術を用いて分化させており、「フットプリントフリー」で機能的かつ高品質な細胞です。
Cat No.
Product Name
Disease StatusDescription
CH-SeV-CW50065
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Healthy Control - (F, 74yr, Caucasian, not Latino)
Healthy Control
Cryopreserved human iPSC-derived cholinergic neurons from healthy control (F, 74)
CH-SeV-CW70067
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Healthy Control - (F, 21yr, Asian)
Healthy Control
Cryopreserved human iPSC-derived cholinergic neurons from healthy control (F, 21)
CH-SeV-CW50023
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Healthy Control - (M, 69yr, Caucasian, not Latino)
Healthy Control
Cryopreserved human iPSC-derived cholinergic neurons from healthy control (M, 69)
CH-SeV-CW10149
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Healthy Control - (M, 38yr, Asian)
Healthy Control
Cryopreserved human iPSC-derived cholinergic neurons from healthy control (M, 38)
CH-SeV-CW50025
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Alzheimer's Disease - (F, 68yr, Caucasian, not Latino)
Alzheimer's Disease
Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 68)
CH-SeV-CW50113
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Alzheimer's Disease - (M, 68yr, Caucasian, not Latino)
Alzheimer's Disease
Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (M, 68)
CH-SeV-CW50114
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Alzheimer's Disease - (F, 72yr, Caucasian, not Latino)
Alzheimer's Disease
Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 72)
CH-SeV-CW50115
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Alzheimer's Disease - (F, 67yr, Caucasian, not Latino)
Alzheimer's Disease
Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 67)
CH-SeV-CW50137
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Alzheimer's Disease - (F, 70yr, Caucasian, not Latino)
Alzheimer's Disease
Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 70)
CH-SeV-CW50147
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Alzheimer's Disease - (M, 71yr, Caucasian, not Latino)
Alzheimer's Disease
Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (M, 71)
CH-SeV-CW50104
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Alzheimer's Disease - (M, 79yr, Caucasian, not Latino)
Alzheimer's Disease
Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (M, 79)
CH-SeV-CW50107
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Alzheimer's Disease - (M, 72yr, Caucasian, not Latino)
Alzheimer's Disease
Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (M, 72)
CH-SeV-CW50169
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Alzheimer's Disease - (F, 68yr, Caucasian, not Latino)
Alzheimer's Disease
Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 68)
CH-SeV-CW50170
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Alzheimer's Disease - (F, 64yr, Caucasian, not Latino)
Alzheimer's Disease
Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 64)
CH-SeV-CW50173
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Alzheimer's Disease - (F, 74yr, Caucasian, not Latino)
Alzheimer's Disease
Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 74)
CH-SeV-CW50174
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Alzheimer's Disease - (F, 76yr, Caucasian, not Latino)
Alzheimer's Disease
Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 76)
CH-SeV-CW50175
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Alzheimer's Disease - (F, 77yr, Caucasian, not Latino)
Alzheimer's Disease
Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 77)
CH-SeV-CW50176
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Alzheimer's Disease - (M, 74yr, Caucasian, not Latino)
Alzheimer's Disease
Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (M, 74)
CH-SeV-CW50177
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Alzheimer's Disease - (F, 85yr, Caucasian, Latino)
Alzheimer's Disease
Cryopreserved human iPSC-derived cholinergic neurons from Alzheimer's Disease patient (F, 85)
CH-SeV-CW60130
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Epilepsy - (F, 6yr, Caucasian, not Latino)
Epilepsy
Cryopreserved human iPSC-derived cholinergic neurons from Epilepsy patient (F, 6)
CH-SeV-CW60231
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Epilepsy - (M, 6yr, Caucasian, not Latino)
Epilepsy
Cryopreserved human iPSC-derived cholinergic neurons from Epilepsy patient (M, 6)
CH-SeV-CW60236
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Epilepsy - (F, 11yr, Caucasian, not Latino)
Epilepsy
Cryopreserved human iPSC-derived cholinergic neurons from Epilepsy patient (F, 11)
CH-SeV-CW20026
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Autism Spectrum Disorder - (M, 10yr, Caucasian, not Latino)
Autism Spectrum Disorder
Cryopreserved human iPSC-derived cholinergic neurons from Autism Spectrum Disorder patient (M, 10)
CH-SeV-CW20090
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Autism Spectrum Disorder - (F, 18yr, Asian)
Autism Spectrum Disorder
Cryopreserved human iPSC-derived cholinergic neurons from Autism Spectrum Disorder patient (F, 18)
CH-SeV-CW10130
Quick-Neuron™ Cholinergic - Human iPSC-derived Neurons Fatty Liver Disease - (M, 36yr, Asian)
Fatty Liver Disease
Cryopreserved human iPSC-derived cholinergic neurons from Fatty Liver Disease patient (M, 36)
よくあるご質問
エリクサジェン・サイエンティフィック社の分化細胞および分化誘導試薬キットを使用するには、ライセンスが必要ですか？
ヒトへの使用や治療・診断目的での使用を除き、エリクサジェン・サイエンティフィック社の分化細胞および分化誘導試薬キットを使用する際に第三者からの追加ライセンスは不要です。
詳細についてはour license statement をご確認ください。
Quick-Neuron™ differentiation kits にはどのようなサイズがありますか？
SmallサイズとLargeサイズをご用意しております。Smallサイズは24ウェルプレートの4ウェル分に使用できる試薬セットで、シングルセル解析、イメージング解析、パイロットスタディなどに最適です。
Largeサイズは6ウェルプレート6ウェル分または10cmディッシュ1枚分に使用できる試薬セットで、ハイスループットスクリーニングやRNAシーケンシングなどのアプリケーションなどに最適です。
更に多くの数量をご希望の場合はお問い合わせください。
